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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant job in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence facts isn't nonetheless obtainable.
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Blocking peptides are peptides that bind specifically into the goal antibody and block antibody binding. These peptide usually includes the epitope regarded by the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the absolute best outcomes are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see which staining is certain.
Goal Info Cyclin E belongs into the hugely conserved cyclin household, whose users are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation styles which lead to your temporal coordination of each and every mitotic event. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress by way of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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